Overview

This kit isolates human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh peripheral blood mononuclear cells (PBMCs) or leukapheresis samples. First, CD25+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells, and unwanted non-Tregs are targeted for depletion. The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications. An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.

Advantages:

• Highly purified human CD4+CD127lowCD25+ Tregs isolated in less than 1 hour• No-wash removal of EasySep™ Releasable RapidSpheres™• Optional isolation of CD4+CD25- responder T cells from the same sample

Components:

EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Catalog #18063)

EasySep™ Human CD25 Positive Selection Cocktail, 1 mL
EasySep™ Human CD127high Depletion Cocktail, 1 mL
EasySep™ Human CD4+ T Cell Enrichment Cocktail, 1 mL
EasySep™ Releasable RapidSpheres™, 1 mL
EasySep™ Dextran RapidSpheres™, 2 x 1 mL
EasySep™ Release Buffer, 2 x 1 mL

RoboSep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Catalog #18063)

EasySep™ Human CD25 Positive Selection Cocktail, 1 mL
EasySep™ Human CD127high Depletion Cocktail, 1 mL
EasySep™ Human CD4+ T Cell Enrichment Cocktail, 1 mL
EasySep™ Releasable RapidSpheres™, 1 mL
EasySep™ Dextran RapidSpheres™, 2 x 1 mL
EasySep™ Release Buffer, 2 x 1 mL
RoboSep™ Buffer (Catalog #20104)
RoboSep™ Filter Tips (Catalog #20125) x 2
EasySep™ EasyTube™-14 (Catalog #20128)

Magnet Compatibility:

• EasySep™ Magnet (Catalog #18000)

• “The Big Easy” EasySep™ Magnet (Catalog #18001)

• EasyEights™ EasySep™ Magnet (Catalog #18103)

• Easy 50 EasySep™ Magnet (Catalog #18002)

• RoboSep™-S (Catalog #21000)

Subtype:

Cell Isolation Kits

Cell Type:

T Cells; T Cells, CD4+; T Cells, Regulatory

Species:

Human

Sample Source:

Leukapheresis; PBMC

Selection Method:

Positive

Application:

Cell Isolation

Brand:

EasySep; RoboSep

Area of Interest:

Immunology

品牌介绍

STEMCELL

STEMCELL Technologies 成立于 1993 年,是加拿大最大的生物科技公司,为生命科学研究领域提供专业的细胞培养基和细胞分选产品。

STEMCELL Technologies Inc. is a Canadian biotechnology company that develops specialty cell culture media, cell isolation systems and accessory products for life science research. Driven by science and a passion for quality, STEMCELL supports the advancement of scientific research around the world with our catalogue of more than 2000 cell biology research tools.

产品列表：

No.	品牌	货号	名称	规格
1	stemcell	60026	Anti-Dextran Antibody, Clone DX1	100ug
2	stemcell	60012	Anti-Human CD32 Antibody, Clone IV.3	100ug
3	stemcell	60004PE.1	Anti-Human CD14 Antibody, Clone M5E2	25tests
4	stemcell	60005AD	Anti-Human CD19 Antibody, Clone HIB19	100tests
5	stemcell	60018AD.1	Anti-Human CD45 Antibody, Clone HI30	25tests
6	stemcell	02002	Human IFN-gamma ELISA Kit	2plates
7	stemcell	02006	Human IL-2 ELISA Kit	2plates
8	stemcell	02012	Human IL-10 ELISA Kit	2plates
9	stemcell	02020	Mouse IFN-gamma ELISA Kit	2plates
10	stemcell	02030	Mouse TNF-alpha ELISA Kit	2plates
11	stemcell	01630	Erythropoietin (EPO) ELISA Kit	1kit
12	stemcell	27000	STEMgrid™-6	1grid
13	stemcell	27270	Reversible Strainers	25/box
14	stemcell	27100	35 mm Culture Dishes	10dishes
15	stemcell	20125	RoboSep™Filter Tips	8racks